Characterization of denaturation and renaturation of DNA ...
Heating . Two variable methods (i.e., heating only and heating with cold shock) were applied to denature the DNA by Isotemp ® dry bath incubator (Fisher Scientific). 10 μL of the DNA was transferred to and sealed in each centrifuge tube (VWR, Suwanee, GA, USA). For the heating only method, five groups of triplicate samples were heated at 95°C for 1, 5, 10, 20, …