Differences in larval pesticide tolerance and esterase ...
Larvae were transferred to 2 ml screw-cap bead mill tubes (Fisher) containing 100 μl of lysis buffer (6 M guanidinium chloride, 100 mM Tris, pH 8.5) and four ceramic beads. The samples were homogenized (Precellys 24, Bertin Instruments) at 6500 rpm for 30 s, then centrifuged at 16,000 rcf, 10 min, 4 °C to remove debris.