An introduction to cryo‐FIB‐SEM cross‐sectioning of frozen ...
This paper encompasses the practical insight of how to FIB‐mill and SEM‐image a cross‐section of a frozen hydrated Life Science sample with cryo‐FIB‐SEM. A sample of yeast ( Saccharomyces Cerevisiae ) has been frequently used for practical purposes and to demonstrate the processes, techniques and problems encountered.